IF-P
Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling Heme Oxygenase 1 (HO-1). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Heme Oxygenase 1 (HO-1) (iFluor™ 594) at 1/50 dilution overnight at 4 ℃, washed with PBS. 33258 was used as nuclear counterstain.| Product Name | iFluor™ 594 Conjugated Heme Oxygenase 1 (HO-1) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human HO-1 aa 227-276 / 288. |
| Conjugation | Alexa Fluor? 594 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-P |
| IF-P:1:50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HMOX1 |
|---|---|
| Gene Synonyms | HO-1 HSP32 HMOX1D bK286B10 |
| Gene Full Name | heme oxygenase 1 |
| Gene Summary | Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008] |
| Alternative Names | 32 kD antibody bK286B10 antibody D8Wsu38e antibody heat shock protein 32 kD antibody heat shock protein 32kD antibody Heat shock protein antibody Heme oxygenase (decycling) 1 antibody Heme oxygenase 1 antibody Hemox antibody HMOX 1 antibody 32 kD antibody bK286B10 antibody D8Wsu38e antibody heat shock protein 32 kD antibody heat shock protein 32kD antibody Heat shock protein antibody Heme oxygenase (decycling) 1 antibody Heme oxygenase 1 antibody Hemox antibody HMOX 1 antibody Hmox antibody Hmox1 antibody HMOX1_HUMAN antibody HO 1 antibody HO antibody HO-1 antibody HO1 antibody Hsp32 antibody |
| Molecular Weight(MW) | 33kDa |
| Cellular Localization | Microsome, Endoplasmic reticulum membrane. |
IF-P
Immunofluorescence analysis of paraffin-embedded human spleen tissue labeling Heme Oxygenase 1 (HO-1). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Heme Oxygenase 1 (HO-1) (iFluor™ 594) at 1/50 dilution overnight at 4 ℃, washed with PBS. 33258 was used as nuclear counterstain.| Application Notes | IF-P:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
