WB
Western blot analysis of extracts from various samples, using Brachyury / Bry Antibody. Lane 1: PC12(heat shock treatment), blocked with antigen-specific peptides, Lane 2: PC12(heat shock treatment), Lane 3: RAW264.7 cells(serum starvation treatment), Lane 4: Rat lung, Lane 5: Mouse liver.IHC
Brachyury / Bry Antibody at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.ICC/IF
Brachyury / Bry Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to Brachyury / Bry |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human T(Accession O15178), corresponding to amino acid residues S321-L371. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TBXT |
|---|---|
| Gene Synonyms | T TFT SAVA |
| Gene Full Name | T-box transcription factor T |
| Gene Summary | The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies. [provided by RefSeq, Sep 2018] |
| Alternative Names | BRAC_HUMAN Brachyury homolog Brachyury protein Bry MGC104817 Protein T SAVA T T brachyury homolog T brachyury transcription factor T Protein T brachyury homolog (mouse) TFT Transcription factor T |
| Molecular Weight(MW) | 47kDa; 47kDa(Calculated). |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of extracts from various samples, using Brachyury / Bry Antibody. Lane 1: PC12(heat shock treatment), blocked with antigen-specific peptides, Lane 2: PC12(heat shock treatment), Lane 3: RAW264.7 cells(serum starvation treatment), Lane 4: Rat lung, Lane 5: Mouse liver.
IHC
Brachyury / Bry Antibody at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
ICC/IF
Brachyury / Bry Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
