WB
Western blot analysis of TLE 1 on different lysates with Rabbit anti-TLE 1 antibody at 1/1,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Lane 5: Human lung tissue lysate (40 µg/Lane) Lane 6: Mouse lung tissue lysate (40 µg/Lane) Lane 7: Rat lung tissue lysate (40 µg/Lane) Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TLE 1 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of 293T cells labeling TLE 1 with Rabbit anti-TLE 1 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TLE 1 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | TLE 1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:50 ICC:1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TLE1 |
|---|---|
| Gene Synonyms | ESG ESG1 GRG1 TLE-1 |
| Gene Full Name | TLE family member 1, transcriptional corepressor |
| Gene Summary | Enables DNA-binding transcription factor binding activity; identical protein binding activity; and transcription corepressor activity. Involved in negative regulation of anoikis; negative regulation of signal transduction; and regulation of gene expression. Located in cytosol and nucleoplasm. Part of beta-catenin-TCF complex. [provided by Alliance of Genome Resources, Nov 2024] |
| Alternative Names | E(Sp1) homolog antibody Enhancer of split groucho 1 antibody Enhancer of split groucho like protein 1 antibody Enhancer of split groucho-like protein 1 antibody ESG antibody ESG1 antibody GRG1 antibody TLE1 antibody TLE1_HUMAN antibody Transducin like enhancer of split 1 (E(sp1) homolog Drosophila) antibody E(Sp1) homolog antibody Enhancer of split groucho 1 antibody Enhancer of split groucho like protein 1 antibody Enhancer of split groucho-like protein 1 antibody ESG antibody ESG1 antibody GRG1 antibody TLE1 antibody TLE1_HUMAN antibody Transducin like enhancer of split 1 (E(sp1) homolog Drosophila) antibody Transducin like enhancer of split 1 antibody Transducin like enhancer of split 1 homolog of Drosophila E(sp1) antibody Transducin like enhancer protein 1 antibody Transducin-like enhancer protein 1 antibody |
| Molecular Weight(MW) | 83kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of TLE 1 on different lysates with Rabbit anti-TLE 1 antibody at 1/1,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Lane 5: Human lung tissue lysate (40 µg/Lane) Lane 6: Mouse lung tissue lysate (40 µg/Lane) Lane 7: Rat lung tissue lysate (40 µg/Lane) Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TLE 1 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of 293T cells labeling TLE 1 with Rabbit anti-TLE 1 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TLE 1 antibody at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 IHC:1:50 ICC:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
