WB
Western blot analysis of WTAP on different lysates with Rabbit anti-WTAP antibody at 1/1,000 dilution. Lane 1: K562 cell lysate (12.5 µg/Lane) Lane 2: Jurkat cell lysate (10 µg/Lane) Lane 3: Hela cell lysate (10 µg/Lane) Lane 4: HepG2 cell lysate (10 µg/Lane) Lane 5: 293T cell lysate (10 µg/Lane) Lane 6: NIH/3T3 cell lysate (10 µg/Lane) Lane 7: PC-12 cell lysate (10 µg/Lane) Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-WTAP antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling WTAP with Rabbit anti-WTAP antibody at 10μg/mL dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-WTAP antibody at 10μg/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | WTAP Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human WTAP aa 1-300 / 396. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:2000 ICC:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | WTAP |
|---|---|
| Gene Synonyms | Mum2 |
| Gene Full Name | WT1 associated protein |
| Gene Summary | The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012] |
| Alternative Names | DKFZp686F20131 antibody Female-lethal(2)D homolog antibody FL2D_HUMAN antibody hFL(2)D antibody KIAA0105 antibody MGC3925 antibody Mum2 antibody OTTHUMP00000017522 antibody OTTHUMP00000017523 antibody PNAS 132 antibody DKFZp686F20131 antibody Female-lethal(2)D homolog antibody FL2D_HUMAN antibody hFL(2)D antibody KIAA0105 antibody MGC3925 antibody Mum2 antibody OTTHUMP00000017522 antibody OTTHUMP00000017523 antibody PNAS 132 antibody PNAS-132 antibody PNAS132 antibody Pre mRNA splicing regulator WTAP antibody Pre-mRNA-splicing regulator WTAP antibody Putative pre mRNA splicing regulator female lethal 2D homolog antibody Putative pre mRNA splicing regulator female lethal(2D) antibody Putative pre-mRNA splicing regulator female lethal(2D) homolog antibody putative pre-mRNA splicing regulator female-lethal(2D) antibody Wilms tumor 1 associated protein antibody Wilms tumor 1 associating protein antibody Wilms tumor 1-associating protein antibody Wilms' tumor 1 associating protein antibody Wilms' tumour 1-associating protein antibody WT1 associated protein antibody WT1-associated protein antibody WT1-associating protein antibody wtap antibody |
| Molecular Weight(MW) | 44kDa(Observed band size:55kDa) |
| Cellular Localization | Nucleus speckle, Nucleus, nucleoplasm, Cytoplasm |
WB
Western blot analysis of WTAP on different lysates with Rabbit anti-WTAP antibody at 1/1,000 dilution. Lane 1: K562 cell lysate (12.5 µg/Lane) Lane 2: Jurkat cell lysate (10 µg/Lane) Lane 3: Hela cell lysate (10 µg/Lane) Lane 4: HepG2 cell lysate (10 µg/Lane) Lane 5: 293T cell lysate (10 µg/Lane) Lane 6: NIH/3T3 cell lysate (10 µg/Lane) Lane 7: PC-12 cell lysate (10 µg/Lane) Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-WTAP antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling WTAP with Rabbit anti-WTAP antibody at 10μg/mL dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-WTAP antibody at 10μg/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 IHC:1:2000 ICC:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
