WB
Various lysates were subjected to SDS PAGE followed by western blot with RNF20 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using RNF20 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNF20 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).IP
IP result of anti-RNF20 (IP:3ug; Detection: 1:500) with HeLa cells lysate 2000ug.| Product Name | RNF20 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | RNF20 fusion protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:8000 IHC:1:50-1:500 ICC:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.45mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RNF20 |
|---|---|
| Gene Synonyms | BRE1 BRE1A hBRE1 |
| Gene Full Name | ring finger protein 20 |
| Gene Summary | The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). [provided by RefSeq, Feb 2012] |
| Alternative Names | BRE1 BRE1 A BRE1A BRE1-A E3 ubiquitin-protein ligase BRE1A |
| Molecular Weight(MW) | 114 kDa |
| Cellular Localization | Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with RNF20 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using RNF20 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNF20 antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IP
IP result of anti-RNF20 (IP:3ug; Detection: 1:500) with HeLa cells lysate 2000ug.| Application Notes | WB:1:1000-1:8000 IHC:1:50-1:500 ICC:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
