WB
Western blot analysis of IKK-alpha/beta expression in mouse muscle tissue lysates,The lane on the right was treated with the antigen-specific peptide.ICC/IF
IKK alpha/ beta Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Rabbit polyclonal antibody to IKK alpha/ beta |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human CHUK(Accession O15111), corresponding to amino acid residues D154-Y204. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHUK/IKBKB |
|---|---|
| Gene Synonyms | BPS2 IKK1 IKKA IKBKA IKK-1 TCF16 NFKBIKA IKK-alpha IKK2 IKKB IKK-2 IMD15 IMD15A IMD15B NFKBIKB IKK-beta |
| Gene Full Name | component of inhibitor of nuclear factor kappa B kinase complex,inhibitor of nuclear factor kappa B kinase subunit beta |
| Gene Summary | his gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008] |
| Alternative Names | chuk CHUK1 Conserved Helix Loop Helix Ubiquitous Kinase Conserved helix loop ubiquitous kinase Conserved helix-loop-helix ubiquitous kinase I Kappa B Kinase 1 I Kappa B Kinase Alpha I-kappa-B kinase 1 I-kappa-B kinase alpha IkappaB kinase IkB kinase alpha subunit IkBKA IKK 1 IKK A IKK a kinase IKK-A IKK-alpha IKK1 IKKA IKKA_HUMAN Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit Inhibitor of nuclear factor kappa-B kinase subunit alpha NFKBIKA Nuclear Factor Kappa B Inhibitor Kinase Alpha Nuclear factor NF kappa B inhibitor kinase alpha Nuclear factor NF-kappa-B inhibitor kinase alpha Nuclear factor NFkappaB inhibitor kinase alpha Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor TCF-16 TCF16 Transcription factor 16 I kappa B kinase 2 I kappa B kinase beta I-kappa-B kinase 2 I-kappa-B-kinase beta IkBKB IKK beta IKK-B IKK-beta IKK2 IKKB IKKB_HUMAN IMD15 Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta Inhibitor of nuclear factor kappa-B kinase subunit beta NFKBIKB Nuclear factor NF-kappa-B inhibitor kinase beta |
| Molecular Weight(MW) | 85kDa; 85kD,87kD(Calculated). |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Western blot analysis of IKK-alpha/beta expression in mouse muscle tissue lysates,The lane on the right was treated with the antigen-specific peptide.
ICC/IF
IKK alpha/ beta Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
