WB
Western blot analysis of SCP3/SYCP3 using anti-SCP3/SYCP3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HepG2 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: C6 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SCP3/SYCP3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for SCP3/SYCP3 at approximately 28 kDa. The expected band size for SCP3/SYCP3 is at 28 kDa.IHC
IHC analysis of SCP3/SYCP3 using anti-SCP3/SYCP3 antibody. SCP3/SYCP3 was detected in a paraffin-embedded section of human testicular cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SCP3/SYCP3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
IF analysis of SCP3/SYCP3 using anti-SCP3/SYCP3 antibody. SCP3/SYCP3 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-SCP3/SYCP3 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of Jurkat cells using anti-SCP3/SYCP3 antibody. Overlay histogram showing Jurkat cells stained with rabbit anti-SCP3/SYCP3 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCP3/SYCP3 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to SCP3/SYCP3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human SCP3/SYCP3 recombinant protein (Position: E75-F236). Human SYCP3 shares 74.7% and 76.5% amino acid (aa) sequence identity with mouse and rat SYCP3, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYCP3 |
|---|---|
| Gene Synonyms | COR1 SCP3 SPGF4 RPRGL4 |
| Gene Full Name | synaptonemal complex protein 3 |
| Gene Summary | This gene encodes an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2010] |
| Alternative Names | COR1 SCP 3 SCP3 SYCP3 synaptonemal complex protein 3 |
| Molecular Weight(MW) | 28kDa |
| Cellular Localization | Centromere,Chromosome,Nucleus. |
WB
Western blot analysis of SCP3/SYCP3 using anti-SCP3/SYCP3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HepG2 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: C6 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SCP3/SYCP3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for SCP3/SYCP3 at approximately 28 kDa. The expected band size for SCP3/SYCP3 is at 28 kDa.
IHC
IHC analysis of SCP3/SYCP3 using anti-SCP3/SYCP3 antibody. SCP3/SYCP3 was detected in a paraffin-embedded section of human testicular cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-SCP3/SYCP3 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
IF analysis of SCP3/SYCP3 using anti-SCP3/SYCP3 antibody. SCP3/SYCP3 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-SCP3/SYCP3 Antibody at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of Jurkat cells using anti-SCP3/SYCP3 antibody. Overlay histogram showing Jurkat cells stained with rabbit anti-SCP3/SYCP3 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCP3/SYCP3 Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 0.5mg/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
