WB
Western blot analysis of ILF3 on different lysates with Rabbit anti-ILF3 antibody at 1/500 dilution. Lane 1: Rat heart tissue lysate(20 µg/Lane) Lane 2: 293 cell lysates Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 200,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-ILF3 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of SiHa cells labeling ILF3 with Rabbit anti-ILF3 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ILF3 antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.FC
Flow cytometric analysis of ILF3 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).| Product Name | ILF3 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human ILF3 aa 2-51 / 894. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2,000 IHC:1:50-1:200 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ILF3 |
|---|---|
| Gene Synonyms | CBTF DRBF MMP4 MPP4 NF90 NFAR NF110 NF90a NF90b NF90c NFAR2 TCP80 DRBP76 NF110b NFAR-1 NFAR-2 NFAR90 TCP110 MPP4110 NF90ctv NFAR110 MPHOSPH4 NF-AT-90 |
| Gene Full Name | interleukin enhancer binding factor 3 |
| Gene Summary | This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014] |
| Alternative Names | CBTF antibody Double stranded RNA binding protein 76 antibody Double-stranded RNA-binding protein 76 antibody DRBF antibody DRBP76 antibody ILF3 antibody ILF3_HUMAN antibody Interleukin enhancer binding factor 3 antibody Interleukin enhancer-binding factor 3 antibody M phase phosphoprotein 4 antibody CBTF antibody Double stranded RNA binding protein 76 antibody Double-stranded RNA-binding protein 76 antibody DRBF antibody DRBP76 antibody ILF3 antibody ILF3_HUMAN antibody Interleukin enhancer binding factor 3 antibody Interleukin enhancer-binding factor 3 antibody M phase phosphoprotein 4 antibody M-phase phosphoprotein 4 antibody MPHOSPH4 antibody MPP4 antibody NF AT 90 antibody NF-AT-90 antibody NF110 antibody NF90 antibody NFAR antibody Nuclear factor associated with dsRNA antibody Nuclear factor of activated T cells 90 kDa antibody Nuclear factor of activated T-cells 90 kDa antibody TCP80 antibody Translational control protein 80 antibody |
| Molecular Weight(MW) | 75/76/77/83/95/96kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Western blot analysis of ILF3 on different lysates with Rabbit anti-ILF3 antibody at 1/500 dilution. Lane 1: Rat heart tissue lysate(20 µg/Lane) Lane 2: 293 cell lysates Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 200,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-ILF3 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of SiHa cells labeling ILF3 with Rabbit anti-ILF3 antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ILF3 antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
FC
Flow cytometric analysis of ILF3 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 ICC:1:50-1:200 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
