WB
K-562 cells were subjected to SDS PAGE followed by western blot with ETV4 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin embedded human stomach cancer tissue slide using ETV4 antibody at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETV4 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti Rabbit IgG(H+L), CL594-Phalloidin (red).FC
1x10^6 U2OS cells were intracellularly stained with 0.25 ug ETV4 Recombinant antibody and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.| Product Name | ETV4 Recombinant antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | ETV4 Fusion Protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:5000-1:50000 IHC:1:200-1:800 ICC:1:200-1:800 FC:0.25ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ETV4 |
|---|---|
| Gene Synonyms | E1AF PEA3 E1A-F PEAS3 |
| Gene Full Name | ETS variant transcription factor 4 |
| Gene Summary | Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in positive regulation of keratinocyte differentiation and positive regulation of transcription by RNA polymerase II. Located in chromosome and nucleolus. [provided by Alliance of Genome Resources, Nov 2024] |
| Alternative Names | PEA3 ETS translocation variant 4 E1A-F E1AF Adenovirus E1A enhancer-binding protein |
| Molecular Weight(MW) | 54kDa(Observed Molecular Weight 61-65kDa) |
| Cellular Localization | Nucleus. |
WB
K-562 cells were subjected to SDS PAGE followed by western blot with ETV4 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin embedded human stomach cancer tissue slide using ETV4 antibody at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETV4 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti Rabbit IgG(H+L), CL594-Phalloidin (red).
FC
1x10^6 U2OS cells were intracellularly stained with 0.25 ug ETV4 Recombinant antibody and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.| Application Notes | WB:1:5000-1:50000 IHC:1:200-1:800 ICC:1:200-1:800 FC:0.25ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
