WB
Western blot analysis of REIIBP/NSD2 on different lysates with Rabbit anti-REIIBP/NSD2 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Neuro-2a cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded Rat testis tissue with Rabbit anti-REIIBP/NSD2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | REIIBP/NSD2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human NSD2 385-584 / 584 (O96028-4). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NSD2 |
|---|---|
| Gene Synonyms | WHS TRX5 KMT3F KMT3G MMSET RAUST WHSC1 REIIBP |
| Gene Full Name | nuclear receptor binding SET domain protein 2 |
| Gene Summary | This gene encodes a protein that contains four domains present in other developmental proteins: a PWWP domain, an HMG box, a SET domain, and a PHD-type zinc finger. It is expressed ubiquitously in early development. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and has also been involved in the chromosomal translocation t(4;14)(p16.3;q32.3) in multiple myelomas. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. Some transcript variants are nonsense-mediated mRNA (NMD) decay candidates, hence not represented as reference sequences. [provided by RefSeq, Jul 2008] |
| Alternative Names | FLJ23286 antibody IL5 promoter REII region binding protein antibody KIAA1090 antibody MGC176638 antibody MMSET antibody MMSET type II antibody Multiple myeloma SET domain containing protein type III antibody Multiple myeloma SET domain protein antibody Multiple myeloma SET domain-containing protein antibody NSD 2 antibody FLJ23286 antibody IL5 promoter REII region binding protein antibody KIAA1090 antibody MGC176638 antibody MMSET antibody MMSET type II antibody Multiple myeloma SET domain containing protein type III antibody Multiple myeloma SET domain protein antibody Multiple myeloma SET domain-containing protein antibody NSD 2 antibody NSD2 antibody NSD2_HUMAN antibody Nuclear receptor binding SET domain protein 2 antibody Nuclear SET domain-containing protein 2 antibody Probable histone-lysine N-methyltransferase NSD2 antibody Protein trithorax-5 antibody REIIBP antibody Trithorax/ash1 related protein 5 antibody TRX5 antibody TRX5 protein antibody WHS antibody Whsc1 antibody Wolf Hirschhorn syndrome candidate 1 antibody Wolf Hirschhorn syndrome candidate 1 protein antibody Wolf-Hirschhorn syndrome candidate 1 protein antibody |
| Molecular Weight(MW) | 66kDa |
| Cellular Localization | Cytoplasm, nucleus, nucleolus. |
WB
Western blot analysis of REIIBP/NSD2 on different lysates with Rabbit anti-REIIBP/NSD2 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Neuro-2a cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded Rat testis tissue with Rabbit anti-REIIBP/NSD2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
