WB
Lymph node (Mouse) Lysate at 40 ug Lymph node (Rat) Lysate at 40 ug Primary: Anti- CD62L at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD. Observed band size: 43 kD.IHC
rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-L-Selectin Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.IF-P
Rat colitis tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-L-Selectin Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei.| Product Name | CD62L Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CD62L: 301-372/372 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 IF-P:1:100-1:500 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Sell |
|---|---|
| Gene Synonyms | Lnhr CD62L LAM-1 Ly-22 Lyam1 LECAM1 Ly-m22 Lyam-1 LECAM-1 L-selectin |
| Gene Full Name | selectin, lymphocyte |
| Gene Summary | Enables cell adhesion molecule binding activity. Acts upstream of or within response to ATP. Located in external side of plasma membrane. Is expressed in brain; brainstem; submandibular gland epithelium; and testis. Used to study type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in Crohn's disease; IgA glomerulonephritis; and ulcerative colitis. Orthologous to human SELL (selectin L). [provided by Alliance of Genome Resources, Dec 2024] |
| Alternative Names | CD62L L-selectin LAM-1 LECAM-1 LECAM1 Lnhr Ly-22 Ly-m22 Lyam-1 Lyam1 LYAM1_MOUSE Sell CD62 antigen-like family member L Leukocyte adhesion molecule 1 (LAM-1) Leukocyte-endothelial cell adhesion molecule 1 (LECAM1) Lymph node homing receptor Lymphocyte antigen 22 (Ly-22) Lymphocyte surface MEL-14 antigen Ly22 LAM1 LEU8 LSEL PLNHR TQ1 LYAM1_HUMAN Leukocyte surface antigen Leu-8 gp90-MEL selectin L lymphocyte adhesion molecule 1 |
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
WB
Lymph node (Mouse) Lysate at 40 ug Lymph node (Rat) Lysate at 40 ug Primary: Anti- CD62L at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD. Observed band size: 43 kD.
IHC
rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-L-Selectin Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
IF-P
Rat colitis tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-L-Selectin Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 IF-P:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
