WB
Western blot analysis of Integrin beta 1 on different lysates with Rabbit anti-Integrin beta 1 antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Integrin beta 1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Immunofluorescence analysis of paraffin-embedded mouse skin tissue labeling Integrin beta 1 with Rabbit anti-Integrin beta 1 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Product Name | Integrin beta 1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Integrin beta-1 aa 652-701 / 798. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:5000 IHC:1:200-1:1000 IF-P:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ITGB1 |
|---|---|
| Gene Synonyms | CD29 FNRB MDF2 VLAB GPIIA MSK12 VLA-BETA |
| Gene Full Name | integrin subunit beta 1 |
| Gene Summary | Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | beta1 integrin antibody CD29 antibody Fibronectin receptor subunit beta antibody FNRB antibody Glycoprotein IIa antibody GP IIa antibody GPIIA antibody Integrin beta-1 antibody integrin VLA-4 beta subunit antibody Integrin beta 1 (fibronectin receptor beta polypeptide antigen CD29 includes MDF2 MSK12) antibody beta1 integrin antibody CD29 antibody Fibronectin receptor subunit beta antibody FNRB antibody Glycoprotein IIa antibody GP IIa antibody GPIIA antibody Integrin beta-1 antibody integrin VLA-4 beta subunit antibody Integrin beta 1 (fibronectin receptor beta polypeptide antigen CD29 includes MDF2 MSK12) antibody ITB1_HUMAN antibody ITGB1 antibody MDF2 antibody MSK12 antibody OTTHUMP00000019420 antibody Very late activation protein beta polypeptide antibody VLA BETA antibody VLA-4 subunit beta antibody VLA-BETA antibody VLAB antibody VLAbeta antibody |
| Molecular Weight(MW) | 88kDa(Observed band size: 120-140 kDa) |
| Cellular Localization | Cell membrane, Cell projection, Cleavage furrow. |
WB
Western blot analysis of Integrin beta 1 on different lysates with Rabbit anti-Integrin beta 1 antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Integrin beta 1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Immunofluorescence analysis of paraffin-embedded mouse skin tissue labeling Integrin beta 1 with Rabbit anti-Integrin beta 1 antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:5000 IHC:1:200-1:1000 IF-P:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
