WB
Various lysates were subjected to SDS PAGE followed by western blot with ARNT/HIF-1 beta antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin embedded mouse embryo tissue slide using ARNT/HIF-1 beta Antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using ARNT/HIF-1 beta antibody at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | ARNT/HIF-1 beta Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | ARNT,HIF1B Fusion Protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:500 ICC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARNT |
|---|---|
| Gene Synonyms | ARNT1 HIF1B TANGO bHLHe2 HIF1BETA HIF-1beta HIF1-beta HIF-1-beta |
| Gene Full Name | aryl hydrocarbon receptor nuclear translocator |
| Gene Summary | This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013] |
| Alternative Names | ARNT HIF-1 beta HIF1B bHLHe2 Class E basic helix-loop-helix protein 2 |
| Molecular Weight(MW) | 86 kDa |
| Cellular Localization | Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with ARNT/HIF-1 beta antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin embedded mouse embryo tissue slide using ARNT/HIF-1 beta Antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using ARNT/HIF-1 beta antibody at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:500 ICC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
