WB
Western Blot analysis of various cells(1 mouse lung 2 mouse brain 3 mouse spleen 4 mouse kindney 5 mouse heart 6 293T 7 hela ) using Antibody diluted at 1:1000. Secondary antibodywas diluted at 1:20000.IHC
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,CREB-1 (phospho Ser133) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.ICC/IF
Immunofluorescence analysis of Hela cell. 1,CREB-1 (phospho Ser133) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-Tubulin Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).| Product Name | CREB-1 (phospho Ser133) Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser133. AA range:100-149. |
| Modification | p-Ser133 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CREB1 |
|---|---|
| Gene Synonyms | CREB CREB-1 |
| Gene Full Name | cAMP responsive element binding protein 1 |
| Gene Summary | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016] |
| Target | Phospho-CREB-1 (S133) Polyclonal Antibody detects endogenous levels of CREB-1 protein only when phosphorylated at S133. |
| Alternative Names | CREB1 Cyclic AMP-responsive element-binding protein 1 CREB-1 cAMP-responsive element-binding protein 1 |
| Molecular Weight(MW) | 37kDa |
| Cellular Localization | Nucleus . |
WB
Western Blot analysis of various cells(1 mouse lung 2 mouse brain 3 mouse spleen 4 mouse kindney 5 mouse heart 6 293T 7 hela ) using Antibody diluted at 1:1000. Secondary antibodywas diluted at 1:20000.
IHC
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,CREB-1 (phospho Ser133) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,CREB-1 (phospho Ser133) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-Tubulin Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C). |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
