WB
Western blot analysis of extracts from various samples, using Collagen X Antibody. Lane 1: Hepg2 cells(serum starvation treatment), blocked with antigen-specific peptides. Lane 2: Hepg2 cells(serum starvation treatment). Lane 3: Hela cells(lps 4h treatment). Lane 4: K562 cells(serum starvation treatment).IHC
Collagen X Antibody at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Product Name | Rabbit polyclonal antibody to Collagen X |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human COL10A1(Accession Q03692), corresponding to amino acid residues A636-M680. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC::1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL10A1 |
|---|---|
| Gene Synonyms | COAA1 COL10A1 collagen type X alpha 1 chain |
| Gene Full Name | collagen type X alpha 1 chain |
| Gene Summary | This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). [provided by RefSeq, Jul 2008] |
| Alternative Names | COAA1_HUMAN Col10a 1 COL10A1 Collagen alpha 1(X) chain Collagen alpha-1(X) chain Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) Collagen type X alpha 1 Collagen X alpha 1 polypeptide CollagenX fa66d11 fb10c08 OTTHUMP00000040411 Procollagen type X alpha 1 Schmid metaphyseal chondrodysplasia wu:fa66d11 wu:fb10c08 |
| Molecular Weight(MW) | 66kDa |
| Cellular Localization | Extracellular matrix, Secreted |
WB
Western blot analysis of extracts from various samples, using Collagen X Antibody. Lane 1: Hepg2 cells(serum starvation treatment), blocked with antigen-specific peptides. Lane 2: Hepg2 cells(serum starvation treatment). Lane 3: Hela cells(lps 4h treatment). Lane 4: K562 cells(serum starvation treatment).
IHC
Collagen X Antibody at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:500-1:2000 IHC::1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
