WB
Western blot analysis of lysates from Mouse liver using CD24 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.Exposure time:30s.ICC/IF
Confocal imaging of Neuro-2a cells using CD24 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue).objective: 100x.IF-P
Confocal imaging of paraffin-embedded Mouse speeln tissue using CD24 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L)(dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.| Product Name | CD24 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 27-53 of mouse CD24 (NP_033976.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PWB |
| WB:1:1000-1:4000 ICC:1:200-1:2000 IF-P:1:200-1:2000 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Cd24a |
|---|---|
| Gene Synonyms | HSA Cd24 Ly-52 nectadrin |
| Gene Full Name | CD24a antigen |
| Gene Summary | Enables Hsp70 protein binding activity; Hsp90 protein binding activity; and carbohydrate binding activity. Involved in several processes, including chemokine receptor transport out of membrane raft; negative regulation of cytokine production; and regulation of inflammatory response to wounding. Acts upstream of or within several processes, including positive regulation of immune response; regulation of cell-cell adhesion; and regulation of lymphocyte activation. Located in several cellular components, including cell projection membrane; external side of plasma membrane; and membrane raft. Is expressed in several structures, including extraembryonic component; forebrain; genitourinary system; hemolymphoid system; and retina. Human ortholog(s) of this gene implicated in multiple sclerosis. Orthologous to human CD24 (CD24 molecule). [provided by Alliance of Genome Resources, Dec 2024] |
| Alternative Names | HSA Cd24 Ly-52 nectadrin |
| Molecular Weight(MW) | 8kDa(Observed MW 37kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of lysates from Mouse liver using CD24 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.Exposure time:30s.
ICC/IF
Confocal imaging of Neuro-2a cells using CD24 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue).objective: 100x.
IF-P
Confocal imaging of paraffin-embedded Mouse speeln tissue using CD24 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L)(dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.| Application Notes | WB:1:1000-1:4000 ICC:1:200-1:2000 IF-P:1:200-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
