WB
Western blot analysis of lysates from Mouse lung, using SMMHC/MYH11 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.Exposure time: 3min.IHC
Immunohistochemistry analysis of paraffin embedded Rat colon tissue using SMMHC/MYH11 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embedded Rat small intestine using SMMHC/MYH11 Rabbit mAb (dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.| Product Name | SMMHC/MYH11 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1160-1313 of human SMMHC/MYH11 (NP_002465.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:1000 IHC:1:500-1:5000 IF-P:1:400-1:1600 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYH11 |
|---|---|
| Gene Synonyms | AAT4 FAA4 SMHC SMMHC VSCM2 SMMS-1 |
| Gene Full Name | myosin heavy chain 11 |
| Gene Summary | The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. A chromosomal rearrangement involving this gene is associated with acute myeloid leukemia of the M4Eo subtype. Mutations in this gene are associated with visceral myopathy, megacystis-microcolon-intestinal hypoperistalsis syndrome 2, and familial thoracic aortic aneurysm 4. [provided by RefSeq, May 2022] |
| Alternative Names | AAT4 FAA4 SMHC SMMHC VSCM2 SMMS-1 SMMHC/MYH11 |
| Molecular Weight(MW) | 227kDa(Observed MW 257kDa) |
| Cellular Localization | Cytoplasm,Melanosome. |
WB
Western blot analysis of lysates from Mouse lung, using SMMHC/MYH11 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.Exposure time: 3min.
IHC
Immunohistochemistry analysis of paraffin embedded Rat colon tissue using SMMHC/MYH11 Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embedded Rat small intestine using SMMHC/MYH11 Rabbit mAb (dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.| Application Notes | WB:1:500-1:1000 IHC:1:500-1:5000 IF-P:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
