WB
Western blot analysis of Bak on different lysates with Rabbit anti-Bak antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: AGS cell lysate Lane 3: HEK-293 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Bak antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
Bak was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-Bak antibody at 2µg/25 µl agarose. Western blot was performed from the immunoprecipitate usingRabbit anti-Bak antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2:Rabbit anti-Bak antibody IP in HeLa cell lysate Lane 3: Rabbit IgG instead of Rabbit anti-Bak antibody in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute.| Product Name | Bak Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Bak aa 1-50 / 211. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000-1:2000 IHC:1:500-1:1000 IP:1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BAK1 |
|---|---|
| Gene Synonyms | AK CDN1 BCL2L7 BAK-LIKE |
| Gene Full Name | BCL2 antagonist/killer 1 |
| Gene Summary | The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008] |
| Alternative Names | Apoptosis regulator BAK antibody BAK antibody BAK like antibody Bak NT antibody BAK_HUMAN antibody Bak1 antibody Bcl 2 homologous antagonist/killer antibody Bcl 2 like 7 protein antibody Bcl-2 homologous antagonist/killer antibody Bcl-2-like protein 7 antibody Apoptosis regulator BAK antibody BAK antibody BAK like antibody Bak NT antibody BAK_HUMAN antibody Bak1 antibody Bcl 2 homologous antagonist/killer antibody Bcl 2 like 7 protein antibody Bcl-2 homologous antagonist/killer antibody Bcl-2-like protein 7 antibody BCL2 antagonist/killer 1 antibody Bcl2 like 7 Protein antibody Bcl2-L-7 antibody BCL2L7 antibody CDN1 antibody Cell death inhibitor 1 antibody MGC117255 antibody MGC3887 antibody NBak antibody Pro apoptotic protein BAK antibody |
| Molecular Weight(MW) | 23kDa |
| Cellular Localization | Mitochondrion membrane. |
WB
Western blot analysis of Bak on different lysates with Rabbit anti-Bak antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: AGS cell lysate Lane 3: HEK-293 cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Bak antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
Bak was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-Bak antibody at 2µg/25 µl agarose. Western blot was performed from the immunoprecipitate usingRabbit anti-Bak antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2:Rabbit anti-Bak antibody IP in HeLa cell lysate Lane 3: Rabbit IgG instead of Rabbit anti-Bak antibody in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute.| Application Notes | WB:1:1000-1:2000 IHC:1:500-1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
