WB
Western blot analysis of Fatty Acid Synthase on different lysates with Rabbit anti-Fatty Acid Synthase antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HEK-293 cell lysate, Lane 3: A549 cell lysate, Lane 4: C2C12 cell lysate, Lane 5: L-929 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Fatty Acid Synthase with Rabbit anti-Fatty Acid Synthase antibody at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Fatty Acid Synthase antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of HeLa cells labeling Fatty Acid Synthase. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Fatty Acid Synthase antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Fatty Acid Synthase antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Fatty Acid Synthase antibody at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
Fatty Acid Synthase was immunoprecipitated from 0.2 mg HeLa cell lysate with abbit anti-Fatty Acid Synthase antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using abbit anti-Fatty Acid Synthase antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: abbit anti-Fatty Acid Synthase antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of abbit anti-Fatty Acid Synthase antibody in HeLa cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 6 seconds.| Product Name | Fatty Acid Synthase Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within C-terminal human Fatty Acid Synthase. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:5000 IHC:1:4000-1:8000 ICC/IF:1:100-1:500 FC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FASN |
|---|---|
| Gene Synonyms | FAS OA-519 SDR27X1 |
| Gene Full Name | fatty acid synthase |
| Gene Summary | The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008] |
| Alternative Names | [Acyl-carrier-protein] S acetyltransferase antibody [Acyl-carrier-protein] S malonyltransferase antibody 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody 3-oxoacyl-[acyl-carrier-protein] reductase antibody 3-oxoacyl-[acyl-carrier-protein] synthase antibody Enoyl-[acyl-carrier-protein] reductase antibody FAS antibody FAS_HUMAN antibody FASN antibody Fatty acid synthase antibody [Acyl-carrier-protein] S acetyltransferase antibody [Acyl-carrier-protein] S malonyltransferase antibody 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody 3-oxoacyl-[acyl-carrier-protein] reductase antibody 3-oxoacyl-[acyl-carrier-protein] synthase antibody Enoyl-[acyl-carrier-protein] reductase antibody FAS antibody FAS_HUMAN antibody FASN antibody Fatty acid synthase antibody MGC14367 antibody MGC15706 antibody OA 519 antibody Oleoyl-[acyl-carrier-protein] hydrolase antibody SDR27X1 antibody Short chain dehydrogenase/reductase family 27X member 1 antibody |
| Molecular Weight(MW) | 273kDa |
| Cellular Localization | Cytoplasm, Melanosome. |
WB
Western blot analysis of Fatty Acid Synthase on different lysates with Rabbit anti-Fatty Acid Synthase antibody at 1/5,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HEK-293 cell lysate, Lane 3: A549 cell lysate, Lane 4: C2C12 cell lysate, Lane 5: L-929 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling Fatty Acid Synthase with Rabbit anti-Fatty Acid Synthase antibody at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Fatty Acid Synthase antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of HeLa cells labeling Fatty Acid Synthase. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Fatty Acid Synthase antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Fatty Acid Synthase antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Fatty Acid Synthase antibody at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
Fatty Acid Synthase was immunoprecipitated from 0.2 mg HeLa cell lysate with abbit anti-Fatty Acid Synthase antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using abbit anti-Fatty Acid Synthase antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input), Lane 2: abbit anti-Fatty Acid Synthase antibody IP in HeLa cell lysate, Lane 3: Rabbit IgG instead of abbit anti-Fatty Acid Synthase antibody in HeLa cell lysate, Blocking/Dilution buffer: primary antibody dilution, Exposure time: 6 seconds.| Application Notes | WB:1:5000 IHC:1:4000-1:8000 ICC/IF:1:100-1:500 FC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
