WB
Western blot analysis of CD200 on different lysates with Rabbit anti-CD200 antibody at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 µg/Lane) Lane 2: NCI-H226 cell lysate (20 µg/Lane) Lane 3: Human brain tissue lysate (30 µg/Lane) Lane 4: Human placenta tissue lysate (30 µg/Lane) Lane 5: Human lung tissue lysate (30 µg/Lane) Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD200 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Immunofluorescence analysis of paraffin-embedded human placenta tissue labeling CD200 with Rabbit anti-CD200 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).FC
Flow cytometric analysis of SK-MEL-28 cells labeling CD200. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | CD200 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human CD200 aa 1-259 / 278. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:1000 IHC:1:2000 IF-P:1:200 FC:1:500-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CD200 |
|---|---|
| Gene Synonyms | MRC MOX1 MOX2 OX-2 |
| Gene Full Name | CD200 molecule |
| Gene Summary | This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016] |
| Alternative Names | Antigen identified by monoclonal antibody MRC OX 2 antibody CD200 antibody CD200 antigen antibody CD200 molecule antibody MOX1 antibody MOX2 antibody MRC antibody MRC OX 2 antigen antibody My033 antibody OX 2 antibody Antigen identified by monoclonal antibody MRC OX 2 antibody CD200 antibody CD200 antigen antibody CD200 molecule antibody MOX1 antibody MOX2 antibody MRC antibody MRC OX 2 antigen antibody My033 antibody OX 2 antibody OX 2 membrane glycoprotein precursor antibody OX-2 membrane glycoprotein antibody OX2G antibody OX2G_HUMAN antibody |
| Molecular Weight(MW) | 31kDa(Observed band size: 40-50kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of CD200 on different lysates with Rabbit anti-CD200 antibody at 1/1,000 dilution. Lane 1: SK-MEL-28 cell lysate (20 µg/Lane) Lane 2: NCI-H226 cell lysate (20 µg/Lane) Lane 3: Human brain tissue lysate (30 µg/Lane) Lane 4: Human placenta tissue lysate (30 µg/Lane) Lane 5: Human lung tissue lysate (30 µg/Lane) Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD200 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Immunofluorescence analysis of paraffin-embedded human placenta tissue labeling CD200 with Rabbit anti-CD200 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
FC
Flow cytometric analysis of SK-MEL-28 cells labeling CD200. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 IHC:1:2000 IF-P:1:200 FC:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
