WB
Western blot analysis of Collagen XVII on different lysates with Rabbit anti-Collagen XVII antibody at 1/5,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: Mouse skin tissue lysate (20 µg/Lane) Lane 3: Rat skin tissue lysate (20 µg/Lane) Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Collagen XVII Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human Collagen XVII aa 1313-1420 / 1497. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:5000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | COL17A1 |
|---|---|
| Gene Synonyms | ERED JEB4 BP180 BPA-2 BPAG2 LAD-1 BA16H23.2 |
| Gene Full Name | collagen type XVII alpha 1 chain |
| Gene Summary | This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008] |
| Alternative Names | 180 kDa bullous pemphigoid antigen 2 antibody Alpha 1 type XVII collagen antibody BA16H23.2 antibody BP 180 antibody BP180 antibody BPA 2 antibody BPAG 2 antibody BPAG2 antibody Bullous pemphigoid antigen 2 antibody COL17A1 antibody 180 kDa bullous pemphigoid antigen 2 antibody Alpha 1 type XVII collagen antibody BA16H23.2 antibody BP 180 antibody BP180 antibody BPA 2 antibody BPAG 2 antibody BPAG2 antibody Bullous pemphigoid antigen 2 antibody COL17A1 antibody Collagen 17 antibody Collagen alpha 1 XVII chain antibody Collagen alpha 1(XVII) chain antibody Collagen alpha1 XVII chain antibody Collagen type XVII alpha 1 antibody Collagen XVII alpha 1 polypeptide antibody CollagenXVII antibody Epidermolysis bullosa junctional localisata variant antibody FLJ60881 antibody KIAA0204 antibody LAD 1 antibody LAD1 antibody |
| Molecular Weight(MW) | 148kDa(Observed band size: 130、180kDa) |
| Cellular Localization | Cell junction, Membrane, Secreted. |
WB
Western blot analysis of Collagen XVII on different lysates with Rabbit anti-Collagen XVII antibody at 1/5,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: Mouse skin tissue lysate (20 µg/Lane) Lane 3: Rat skin tissue lysate (20 µg/Lane) Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Collagen XVII antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
