WB
Western blot analysis of Laminnin 5 alpha 3 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.ICC/IF
ICC staining of Laminnin 5 alpha 3 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).| Product Name | Laminin 5 alpha 3 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Laminnin 5 alpha 3 aa 50-150. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:1000 ICC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LAMA3 |
|---|---|
| Gene Synonyms | E170 LOCS BM600 JEB2A JEB2B JEB2C LAMNA |
| Gene Full Name | laminin subunit alpha 3 |
| Gene Summary | The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Alternative Names | E170 antibody Epiligrin 170 kDa subunit antibody Epiligrin subunit alpha antibody Kalinin subunit alpha antibody LAMA3 antibody LAMA3A antibody Laminin 5 alpha3 antibody Laminin A3 antibody laminin alpha 3 antibody laminin alpha3 antibody E170 antibody Epiligrin 170 kDa subunit antibody Epiligrin subunit alpha antibody Kalinin subunit alpha antibody LAMA3 antibody LAMA3A antibody Laminin 5 alpha3 antibody Laminin A3 antibody laminin alpha 3 antibody laminin alpha3 antibody Laminin subunit alpha-3 antibody Laminin-5 subunit alpha antibody Laminin-6 subunit alpha antibody Laminin-7 subunit alpha antibody laminin5 alpha 3 antibody Laminin5 alpha3 antibody LAMNA antibody LOCS antibody Nicein subunit alpha antibody |
| Molecular Weight(MW) | 367kDa |
| Cellular Localization | Secreted. |
WB
Western blot analysis of Laminnin 5 alpha 3 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
ICC/IF
ICC staining of Laminnin 5 alpha 3 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:1000 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
