IHC
Immunohistochemical analysis of paraffin-embedded esophageal cancers tissues using MCM2 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.IHC
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MCM2 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.| Product Name | Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) Used for immunofluorescence staining. The antibody is affinity purified Goat anti Rabbit IgG, which has good immunoreactivity with Rabbit IgG; No cross reactivity with human IgG, mouse IgG, etc. Omnimabs® The dye is a new generation fluorescent probe independently developed by our company, which has the advantages of high fluorescence brightness, good light stability, and low background. Omnimabs® 488 Goat Anti-Rabbit IgG(H&L)for immunofluorescence staining is 1:100-1:1000, which can be adjusted according to the specific situation of the antigen and antibody. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG(H+L) |
| Host Species | Goat |
| Tested Applications | FCICC/IF |
| IHC::200-1:1000 |
|
| Species Reactivity | Rabbit |
| Purification | Affinity purified |
| Target | Ex/Em: 490/515 nm |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded esophageal cancers tissues using MCM2 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
IHC
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MCM2 antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.| Application Notes | IHC::200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 ℃ away from light. To avoid repeated freezing and thawing, this product can be packaged in small quantities. |
| Storage Buffer | 5 mM HEPES sodium solution pH 7.5, 75 mM NaCl, 1 mg/mL BSA, 0.01% PC300, 50% glycerol. |
Data sheet for OM643486