ICC/IF
Immunofluorescence analysis of Hela cells using SV2A antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.ICC/IF
Immunofluorescence analysis of Hela cells using ESE1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Product Name | Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) |
|---|---|
| Antibody Type | Secondary Antibodies |
| Product description | Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) Used for immunofluorescence staining. The antibody is affinity purified Goat anti Rabbit IgG, which has good immunoreactivity with Rabbit IgG; No cross reactivity with human IgG, mouse IgG, etc. Omnimabs® The dye is a new generation fluorescent probe independently developed by our company, which has the advantages of high fluorescence brightness, good light stability, and low background. Omnimabs® 488 Goat Anti-Rabbit IgG(H&L)for immunofluorescence staining is 1:100-1:1000, which can be adjusted according to the specific situation of the antigen and antibody. |
| Conjugation | AF488 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG(H+L) |
| Host Species | Goat |
| Tested Applications | FCICC/IF |
| ICC/IF:1:100-1:500 |
|
| Species Reactivity | Rabbit |
| Purification | Affinity purified |
| Target | Ex/Em: 490/515 nm |
|---|
ICC/IF
Immunofluorescence analysis of Hela cells using SV2A antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
ICC/IF
Immunofluorescence analysis of Hela cells using ESE1 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Application Notes | ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 ℃ away from light. To avoid repeated freezing and thawing, this product can be packaged in small quantities. |
| Storage Buffer | 5 mM HEPES sodium solution pH 7.5, 75 mM NaCl, 1 mg/mL BSA, 0.01% PC300, 50% glycerol. |
