WB
Various lysates were subjected to SDS PAGE followed by western blot with FA2H antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using FA2H antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using FA2H antibody at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | FA2H Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | FA2H fusion protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:250-1:1000 ICC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FA2H |
|---|---|
| Gene Synonyms | FAAH FAH1 SCS7 SPG35 FAXDC1 |
| Gene Full Name | fatty acid 2-hydroxylase |
| Gene Summary | This gene encodes a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups. Mutations in this gene have been associated with leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia.[provided by RefSeq, Mar 2010] |
| Alternative Names | FAXDC1 Fatty acid hydroxylase domain-containing protein 1 Fatty acid alpha-hydroxylase Fatty acid 2-hydroxylase FAAH |
| Molecular Weight(MW) | 43kDa(Observed Molecular Weight 38-43 kDa) |
| Cellular Localization | Endoplasmic reticulum membrane,Microsome membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with FA2H antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using FA2H antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using FA2H antibody at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:2000 IHC:1:250-1:1000 ICC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
