WB
Western blot analysis of anti- MADCAM1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. Use rabbit anti- MADCAM1 1:1000, probed with a goat anti rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.IHC
IHC analysis using anti-MADCAM1 antibody. detected in paraffin-embedded section of human spleen tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis using anti- MADCAM1 antibody. detected in paraffin-embedded section of human appendicitis tissue. The tissue section were stained using the Dylight550 conjugated Anti-rabbit IgG Secondary Antibody (red)and counterstained with DAPI (blue).| Product Name | Anti-MADCAM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human MAdCAM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MADCAM1 |
|---|---|
| Gene Synonyms | MACAM1 |
| Gene Full Name | mucosal vascular addressin cell adhesion molecule 1 |
| Gene Summary | The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
| Alternative Names | hMAdCAM 1 MACAM1 MAdCAM1 |
| Molecular Weight(MW) | 40kDa(Observed MW 60kDa) |
| Cellular Localization | Membrane |
WB
Western blot analysis of anti- MADCAM1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. Use rabbit anti- MADCAM1 1:1000, probed with a goat anti rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.
IHC
IHC analysis using anti-MADCAM1 antibody. detected in paraffin-embedded section of human spleen tissue. Peroxidase Conjugated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis using anti- MADCAM1 antibody. detected in paraffin-embedded section of human appendicitis tissue. The tissue section were stained using the Dylight550 conjugated Anti-rabbit IgG Secondary Antibody (red)and counterstained with DAPI (blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
