WB
Western blot analysis of lysates from A375 cells, using RIPK4 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.IHC
Immunohistochemistry analysis of paraffin embedded Human liver damage using RIPK4 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.IHC
Immunohistochemistry analysis of paraffin embedded Human stomach using RIPK4 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.| Product Name | RIPK4 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 240-520 of human RIPK4 (NP_065690.2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RIPK4 |
|---|---|
| Gene Synonyms | DIK PKK PPS2 RIP4 ANKK2 NKRD3 ANKRD3 CHANDS |
| Gene Full Name | receptor interacting serine/threonine kinase 4 |
| Gene Summary | The protein encoded by this gene is a serine/threonine protein kinase that interacts with protein kinase C-delta. The encoded protein can also activate NFkappaB and is required for keratinocyte differentiation. This kinase undergoes autophosphorylation. [provided by RefSeq, Jul 2008] |
| Alternative Names | DIK PKK PPS2 RIP4 ANKK2 NKRD3 ANKRD3 CHANDS RIPK4 |
| Molecular Weight(MW) | 92kDa |
| Cellular Localization | Cytoplasm,Membrane,Peripheral membrane protein |
WB
Western blot analysis of lysates from A375 cells, using RIPK4 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
IHC
Immunohistochemistry analysis of paraffin embedded Human liver damage using RIPK4 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
IHC
Immunohistochemistry analysis of paraffin embedded Human stomach using RIPK4 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
