WB
Western blot analysis of various lysates, using Claudin 6 Rabbit mAb at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.IHC
Immunohistochemistry analysis of paraffin embedded Human seminoma tissue using Claudin 6 Rabbit mAb at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.| Product Name | Claudin 6 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 119-219 of human Claudin 6(NP_067018.2) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| WB:1:3000-1:12000 IHC:1:500-1:1000 ELISA:Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CLDN6 |
|---|---|
| Gene Full Name | claudin 6 |
| Gene Summary | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. This gene encodes a component of tight junction strands, which is a member of the claudin family. The protein is an integral membrane protein and is one of the entry cofactors for hepatitis C virus. The gene methylation may be involved in esophageal tumorigenesis. This gene is adjacent to another family member CLDN9 on chromosome 16.[provided by RefSeq, Aug 2010] |
| Molecular Weight(MW) | 23kDa(Observed MW 22kDa) |
| Cellular Localization | apicolateral plasma membrane,bicellular tight junction,plasma membrane. |
WB
Western blot analysis of various lysates, using Claudin 6 Rabbit mAb at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
IHC
Immunohistochemistry analysis of paraffin embedded Human seminoma tissue using Claudin 6 Rabbit mAb at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.| Application Notes | WB:1:3000-1:12000 IHC:1:500-1:1000 ELISA:Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
