WB
Western blot analysis of extracts from Rat heart, using CXADR Antibody. The lane on the left was treated with blocking peptide.IHC
CXADR Antibody at 1/100 staining human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.ICC/IF
CXADR Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Rabbit polyclonal antibody to CXADR |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human CXADR(Accession P78310), corresponding to amino acid residues C6-E56. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXADR |
|---|---|
| Gene Synonyms | CAR HCAR CAR4/6 |
| Gene Full Name | CXADR Ig-like cell adhesion molecule |
| Gene Summary | The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011] |
| Alternative Names | 46 kD coxsackievirus and adenovirus receptor CAR protein 46 kD coxsackievirus and adenovirus receptor protein CAR CAR4/6 Coxsackie virus and adenovirus receptor Coxsackie virus B receptor Coxsackievirus and adenovirus receptor Coxsackievirus B adenovirus receptor Coxsackievirus B-adenovirus receptor CVB3 binding protein CVB3-binding protein Cxadr CXAR_HUMAN hCAR HCVADR |
| Molecular Weight(MW) | 40KD |
| Cellular Localization | Cell membrane>Single-pass type I membrane protein. Basolateral cell membrane>Single-pass type I membrane protein. Cell junction>Tight junction. Cell junction>Adherens junction. |
WB
Western blot analysis of extracts from Rat heart, using CXADR Antibody. The lane on the left was treated with blocking peptide.
IHC
CXADR Antibody at 1/100 staining human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-rabbit antibody was used as the secondary antibody.
ICC/IF
CXADR Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
