WB
Western blot analysis of various lysates using Ly-6G Rabbit mAb at 1:11000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): NIH/3T3 Exposure time: 45s.IHC
Immunohistochemistry analysis of paraffin embedded Mouse spleen tissue using Ly-6G Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IF-P
Confocal imaging of paraffin-embedded Mouse spleen tissue using Ly-6G Rabbit mAb (dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. objective: 40x.| Product Name | Ly-6G Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 27-105 of mouse Ly-6G (NP_001297367.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:5000-1:20000 IHC:1:2000-1:8000 ICC:1:400-1:1600 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Ly6g |
|---|---|
| Gene Synonyms | Gr1 Gr-1 Ly-6G |
| Gene Full Name | lymphocyte antigen 6 family member G |
| Gene Summary | Predicted to enable acetylcholine receptor binding activity and acetylcholine receptor inhibitor activity. Predicted to be involved in acetylcholine receptor signaling pathway. Located in external side of plasma membrane. Is expressed in large intestine; small intestine; and spleen. [provided by Alliance of Genome Resources, Dec 2024] |
| Alternative Names | Gr1 Gr-1 Ly-6G |
| Molecular Weight(MW) | 14kDa( Observed MW 20-40kDa) |
| Cellular Localization | external side of plasma membrane. |
WB
Western blot analysis of various lysates using Ly-6G Rabbit mAb at 1:11000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): NIH/3T3 Exposure time: 45s.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse spleen tissue using Ly-6G Rabbit mAb at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IF-P
Confocal imaging of paraffin-embedded Mouse spleen tissue using Ly-6G Rabbit mAb (dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. objective: 40x.| Application Notes | WB:1:5000-1:20000 IHC:1:2000-1:8000 ICC:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.09% sodium azid,0.05% BSA,50% glycerol,pH7.3. |
