WB
Western blot analysis of extracts of various cell lines, using MMP8 an tibody at 1:1000 dilution. Secondary antibody: HRP Goat An ti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.IHC
Immunohistochemistry of paraffin-embedded human liver cancer using MMP8 Rabbit pAb at dilution of 1:150 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.ICC/IF
Immunofluorescent analysis of HepG2 cells using MMP8 antibody at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti Rabbit IgG(H+L).| Product Name | MMP8 polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein of human MMP8(NP_002415.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50 -1:200 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MMP8 |
|---|---|
| Gene Synonyms | HNC CLG1 MMP-8 PMNL-CL |
| Gene Full Name | matrix metallopeptidase 8 |
| Gene Summary | This gene encodes a member of the matrix metalloproteinase (MMP) family of proteins. These proteins are involved in the breakdown of extracellular matrix in embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Proteolysis at different sites on this protein results in multiple active forms of the enzyme with distinct N-termini. This protein functions in the degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015] |
| Molecular Weight(MW) | 60KDa |
| Cellular Localization | Cytoplasmic granule,Secreted, extracellular space, extracellular matrix. |
WB
Western blot analysis of extracts of various cell lines, using MMP8 an tibody at 1:1000 dilution. Secondary antibody: HRP Goat An ti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
IHC
Immunohistochemistry of paraffin-embedded human liver cancer using MMP8 Rabbit pAb at dilution of 1:150 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ICC/IF
Immunofluorescent analysis of HepG2 cells using MMP8 antibody at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti Rabbit IgG(H+L).| Application Notes | WB:1:500-1:2000 IHC:1:50 -1:200 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
