IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CD66b antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD66b antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Fluorescence multiplex immunohistochemical analysis of human cervical carcinoma (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD66b (Green), anti-CD11b (Red) and anti-CD68 (Yellow) on human cervical carcinoma. HRP Conjugated UltraPolymer Goat Polyclonal Antibody was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit. The section was incubated in three rounds of staining: in the order of anti-CD66b (1/1,000 dilution), anti-CD11b (1/1,000 dilution) and anti-CD68 (1/3,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.| Product Name | CD66b Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human CD66b aa 1-250. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHC |
| IHC:1:1000 IF-P:1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CEACAM8 |
|---|---|
| Gene Synonyms | CD67 CGM6 CD66b NCA-95 |
| Gene Full Name | CEA cell adhesion molecule 8 |
| Gene Summary | Enables protein heterodimerization activity. Involved in heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. Located in cell surface and extracellular space. Biomarker of severe acute respiratory syndrome. [provided by Alliance of Genome Resources, Dec 2024] |
| Alternative Names | Carcinoembryonic antigen CGM6 antibody Carcinoembryonic antigen gene family member 6 antibody Carcinoembryonic antigen related cell adhesion molecule 8 antibody Carcinoembryonic antigen-related cell adhesion molecule 8 antibody CD 66b antibody CD 67 antibody CD66b antibody CD66b antigen antibody CD67 antibody CD67 antigen antibody Carcinoembryonic antigen CGM6 antibody Carcinoembryonic antigen gene family member 6 antibody Carcinoembryonic antigen related cell adhesion molecule 8 antibody Carcinoembryonic antigen-related cell adhesion molecule 8 antibody CD 66b antibody CD 67 antibody CD66b antibody CD66b antigen antibody CD67 antibody CD67 antigen antibody CEACAM 8 antibody CEACAM8 antibody CEAM8_HUMAN antibody CGM 6 antibody CGM6 antibody NCA 95 antibody NCA95 antibody Non-specific cross-reacting antigen NCA-95 antibody Nonspecific cross reacting antigen NCA 95 antibody Nonspecific cross reacting antigen NCA95 antibody |
| Cellular Localization | Cell membrane, Cell surface. |
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CD66b antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD66b antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Fluorescence multiplex immunohistochemical analysis of human cervical carcinoma (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD66b (Green), anti-CD11b (Red) and anti-CD68 (Yellow) on human cervical carcinoma. HRP Conjugated UltraPolymer Goat Polyclonal Antibody was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit. The section was incubated in three rounds of staining: in the order of anti-CD66b (1/1,000 dilution), anti-CD11b (1/1,000 dilution) and anti-CD68 (1/3,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.| Application Notes | IHC:1:1000 IF-P:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
