WB
Western blot analysis of various lysates using ACSS1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.IHC
Immunohistochemistry analysis of paraffin embedded Human colon carcinoma using ACSS1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.ICC/IF
Immunofluorescence analysis of L929 cells using ACSS1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | ACSS1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 340-689 of human ACSS1 (NP_115890.2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACSS1 |
|---|---|
| Gene Synonyms | ACAS2L ACECS1 AceCS2L |
| Alternative Names | ACAS2L ACECS1 AceCS2L ACSS1 |
| Molecular Weight(MW) | 75kDa |
| Cellular Localization | Mitochondrion matrix |
WB
Western blot analysis of various lysates using ACSS1 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
IHC
Immunohistochemistry analysis of paraffin embedded Human colon carcinoma using ACSS1 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ICC/IF
Immunofluorescence analysis of L929 cells using ACSS1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
