WB
Western blot analysis of various lysates using TriMethyl-Histone H3-K36 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.IHC
Immunohistochemistry analysis of paraffin embedded Mouse colon tissue using TriMethyl-Histone H3-K36 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.DB
Dot-blot analysis of all sorts of peptides using TriMethyl-Histone H3-K36 antibody at 1:1000 dilution.IP
Immunoprecipitation analysis of 600 μg extracts of 293F cells using 5 μg TriMethyl-Histone H3-K36 antibody. Western blot was performed from the immunoprecipitate using TriMethyl-Histone H3 K36 antibody at a dilution of 1:1000.| Product Name | TriMethyl-Histone H3-K36 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic trimethylated peptide around K36 of human Histone H3 (NP_003520.1). |
| Modification | TriMethyl K36 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBIHCIPWB |
| WB:1:500 - 1:1000 IHC:1:500 - 1:1000 DB::500 - 1:1000 IP::0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3C1,H3-4 |
|---|---|
| Gene Synonyms | H3/A H3C2 H3C3 H3C4 H3C6 H3C7 H3C8 H3FA H3C10 H3C11 H3C12 HIST1H3A |
| Gene Full Name | H3 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3.4 H3/g H3FT H3t HIST3H3 Histone H3 HIST1H3A TriMethyl-Histone H3-K36 |
| Molecular Weight(MW) | 15kDa |
| Cellular Localization | Chromosome, nucleus. |
WB
Western blot analysis of various lysates using TriMethyl-Histone H3-K36 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse colon tissue using TriMethyl-Histone H3-K36 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
DB
Dot-blot analysis of all sorts of peptides using TriMethyl-Histone H3-K36 antibody at 1:1000 dilution.
IP
Immunoprecipitation analysis of 600 μg extracts of 293F cells using 5 μg TriMethyl-Histone H3-K36 antibody. Western blot was performed from the immunoprecipitate using TriMethyl-Histone H3 K36 antibody at a dilution of 1:1000.| Application Notes | WB:1:500 - 1:1000 IHC:1:500 - 1:1000 DB::500 - 1:1000 IP::0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
