WB
Western blot analysis of FBP1 on different lysates with Rabbit anti-FBP1 antibody at 1/1,000 dilution. Lane 1: Human liver tissue lysate, Lane 2: Human lung tissue lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 7 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-FBP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
ICC staining of FBP1 in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).| Product Name | FBP1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human FBP1 aa 244-338 / 338. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:2000 IHC:1:50-1:200 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | FBP1 |
|---|---|
| Gene Synonyms | FBP |
| Gene Full Name | fructose-bisphosphatase 1 |
| Gene Summary | Fructose-1,6-bisphosphatase 1, a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. [provided by RefSeq, Jul 2008] |
| Alternative Names | 6-bisphosphatase 1 antibody 6-bisphosphate 1-phosphohydrolase 1 antibody D fructose 1 6 bisphosphate 1 phosphohydrolase 1 antibody D-fructose-1 antibody EC 3.1.3.11 antibody F16P1_HUMAN antibody FBP antibody FBP 1 antibody FBP1 antibody FBPase 1 antibody 6-bisphosphatase 1 antibody 6-bisphosphate 1-phosphohydrolase 1 antibody D fructose 1 6 bisphosphate 1 phosphohydrolase 1 antibody D-fructose-1 antibody EC 3.1.3.11 antibody F16P1_HUMAN antibody FBP antibody FBP 1 antibody FBP1 antibody FBPase 1 antibody Fructose 1 6 bisphosphatase 1 antibody Fructose bisphosphatase 1 antibody Fructose-1 antibody Growth inhibiting protein 17 antibody Liver fructose bisphosphatase antibody |
| Molecular Weight(MW) | 37kDa |
| Cellular Localization | Cytoplasm. Nucleus. Extracellular exosome. |
WB
Western blot analysis of FBP1 on different lysates with Rabbit anti-FBP1 antibody at 1/1,000 dilution. Lane 1: Human liver tissue lysate, Lane 2: Human lung tissue lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 7 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-FBP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
ICC staining of FBP1 in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:1000-1:2000 IHC:1:50-1:200 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
