WB
Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate, Lane 2: NCI-H226 cell lysate, Lane 3: A549 cell lysate, Lane 4: C2C12 cell lysate, Lane 5: Neuro-2a cell lysate, Lane 6: Human brain tissue lysate, Lane 7: Mouse brain tissue lysate, Lane 8: Rat brain tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | DFNA5 / GSDME Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human GSDME aa 1-496. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GSDME |
|---|---|
| Gene Synonyms | DFNA5 ICERE-1 |
| Gene Full Name | gasdermin E |
| Gene Summary | Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | 2310037D07Rik antibody 4932441K13Rik antibody Deafness autosomal dominant 5 antibody Deafness autosomal dominant 5 protein antibody DFNA5 antibody DFNA5 gene antibody DFNA5_HUMAN antibody Dfna5h antibody EG14210 antibody Fin15 antibody 2310037D07Rik antibody 4932441K13Rik antibody Deafness autosomal dominant 5 antibody Deafness autosomal dominant 5 protein antibody DFNA5 antibody DFNA5 gene antibody DFNA5_HUMAN antibody Dfna5h antibody EG14210 antibody Fin15 antibody ICERE 1 antibody ICERE-1 antibody Inversely correlated with estrogen receptor expression 1 antibody Non-syndromic hearing impairment protein 5 antibody Nonsyndromic hearing impairment protein antibody |
| Molecular Weight(MW) | 53kDa |
| Cellular Localization | Cytoplasm, cytosol. |
WB
Western blot analysis of DFNA5 / GSDME on different lysates with Rabbit anti-DFNA5 / GSDME antibody at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate, Lane 2: NCI-H226 cell lysate, Lane 3: A549 cell lysate, Lane 4: C2C12 cell lysate, Lane 5: Neuro-2a cell lysate, Lane 6: Human brain tissue lysate, Lane 7: Mouse brain tissue lysate, Lane 8: Rat brain tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
