WB
Western blot analysis of LPCAT3 on different lysates with Rabbit anti-LPCAT3 antibody at 1/2,000 dilution. Lane 1: HT-29 cell lysate, Lane 2: HepG2 cell lysate, Lane 3: HeLa cell lysate, Lane 4: COS-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling LPCAT3 with Rabbit anti-LPCAT3 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LPCAT3 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | LPCAT3 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human LPCAT3 aa 1-363. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:2000 ICC:1:100 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LPCAT3 |
|---|---|
| Gene Synonyms | C3F LPCAT LPSAT OACT5 nessy MBOAT5 LPLAT 5 LPLAT12 |
| Gene Full Name | lysophosphatidylcholine acyltransferase 3 |
| Gene Summary | Enables 1-acylglycerophosphocholine O-acyltransferase activity; 1-acylglycerophosphoethanolamine O-acyltransferase activity; and 1-acylglycerophosphoserine O-acyltransferase activity. Involved in phosphatidylcholine acyl-chain remodeling; phosphatidylethanolamine acyl-chain remodeling; and phosphatidylserine acyl-chain remodeling. Located in endoplasmic reticulum membrane. [provided by Alliance of Genome Resources, Jan 2025] |
| Alternative Names | 1-acylglycerophosphocholine O-acyltransferase antibody 1-acylglycerophosphoserine O-acyltransferase antibody LPCAT antibody Lpcat3 antibody LPLAT 5 antibody LPSAT antibody Lyso-PC acyltransferase 3 antibody Lyso-PC acyltransferase antibody Lyso-PS acyltransferase antibody Lysophosphatidylcholine acyltransferase 3 antibody 1-acylglycerophosphocholine O-acyltransferase antibody 1-acylglycerophosphoserine O-acyltransferase antibody LPCAT antibody Lpcat3 antibody LPLAT 5 antibody LPSAT antibody Lyso-PC acyltransferase 3 antibody Lyso-PC acyltransferase antibody Lyso-PS acyltransferase antibody Lysophosphatidylcholine acyltransferase 3 antibody Lysophosphatidylcholine acyltransferase antibody Lysophosphatidylserine acyltransferase antibody Lysophospholipid acyltransferase 5 antibody MBOA5 antibody MBOA5_HUMAN antibody Membrane-bound O-acyltransferase domain-containing protein 5 antibody O-acyltransferase domain-containing protein 5 antibody |
| Molecular Weight(MW) | 56kDa(Observed band size: 45 kDa) |
| Cellular Localization | Endoplasmic reticulum membrane. |
WB
Western blot analysis of LPCAT3 on different lysates with Rabbit anti-LPCAT3 antibody at 1/2,000 dilution. Lane 1: HT-29 cell lysate, Lane 2: HepG2 cell lysate, Lane 3: HeLa cell lysate, Lane 4: COS-1 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling LPCAT3 with Rabbit anti-LPCAT3 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LPCAT3 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:2000 ICC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
