WB
Western blot analysis of Phospho-RPS6 (S235 + S236) on different lysates with Rabbit anti-Phospho-RPS6 (S235 + S236) antibody at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: Hela cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Phospho-RPS6 (S235 + S236) antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
mmunocytochemistry analysis of Hela cells labeling Phospho-RPS6 (S235 + S236) with Rabbit anti-Phospho-RPS6 (S235 + S236) antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-RPS6 (S235 + S236) antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.| Product Name | Phospho-RPS6 (S235 + S236) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human RPS6 aa 200 to the C-terminus (phospho S235 + S236). |
| Modification | p-S235 + S236 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:200 ICC:1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RPS6 |
|---|---|
| Gene Synonyms | S6 eS6 |
| Gene Full Name | ribosomal protein S6 |
| Gene Summary | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| Alternative Names | 40S ribosomal protein S6 antibody Air8 antibody NP33 antibody Phosphoprotein NP33 antibody Pp30 antibody Ribosomal protein S6 antibody RP S6 antibody rps6 antibody RS6 antibody RS6_HUMAN antibody 40S ribosomal protein S6 antibody Air8 antibody NP33 antibody Phosphoprotein NP33 antibody Pp30 antibody Ribosomal protein S6 antibody RP S6 antibody rps6 antibody RS6 antibody RS6_HUMAN antibody S6 antibody S6 Ribosomal Protein antibody |
| Molecular Weight(MW) | 29kDa(Observed band size: 33kDa) |
| Cellular Localization | Cytosol, Endoplasmic reticulum, Nucleus. |
WB
Western blot analysis of Phospho-RPS6 (S235 + S236) on different lysates with Rabbit anti-Phospho-RPS6 (S235 + S236) antibody at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: Hela cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Phospho-RPS6 (S235 + S236) antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
mmunocytochemistry analysis of Hela cells labeling Phospho-RPS6 (S235 + S236) with Rabbit anti-Phospho-RPS6 (S235 + S236) antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-RPS6 (S235 + S236) antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.| Application Notes | WB:1:1000 IHC:1:200 ICC:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
