WB
Western blotting analysis. A: Nthy-ori3-1 cell; B: TPC cell; C: IHH-4 cell; D: BCPAP cell.IHC
MLL2 Antibody at 1/100 staining human Prostate carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.ICC/IF
MLL2 Antibody staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to MLL2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human KMT2D(Accession O14686), corresponding to amino acid residues G1967-T2017. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:3000 IHC:1:50-1:200 ICC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KMT2D |
|---|---|
| Gene Synonyms | ALR KMS MLL2 MLL4 AAD10 BCAHH KABUK1 TNRC21 CAGL114 |
| Gene Summary | lysine methyltransferase 2D |
| Target Description | The protein encoded by this gene is a histone methyltransferase that methylates the Lys-4 position of histone H3. The encoded protein is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the beta-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome. [provided by RefSeq, Oct 2010] |
| Alternative Names | AAD10 ALL1 related gene ALL1-related protein ALR CAGL114 Histone-lysine N-methyltransferase MLL2 KABUK1 Kabuki make up syndrome Kabuki mental retardation syndrome KMS KMT2B KMT2D Lysine N methyltransferase 2D Lysine N-methyltransferase 2B MLL2 MLL2_HUMAN MLL4 Myeloid/lymphoid or mixed lineage leukemia 2 Myeloid/lymphoid or mixed-lineage leukemia protein 2 TNRC21 Trinucleotide repeat containing 21 |
| Molecular Weight(MW) | 593kDa |
| Cellular Localization | Nucleus. |
WB
Western blotting analysis. A: Nthy-ori3-1 cell; B: TPC cell; C: IHH-4 cell; D: BCPAP cell.
IHC
MLL2 Antibody at 1/100 staining human Prostate carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
ICC/IF
MLL2 Antibody staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:1000-1:3000 IHC:1:50-1:200 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
