WB
Western blot analysis of extracts from Rat brain, using GABRA2 Antibody. The lane on the left was treated with blocking peptide.ICC/IF
GABRA2 Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Rabbit polyclonal antibody to GABRA2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human GABRA2(Accession P47869), corresponding to amino acid residues N9-P59. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GABRA2 |
|---|---|
| Gene Synonyms | DEE78 EIEE78 |
| Gene Full Name | gamma-aminobutyric acid type A receptor subunit alpha2 |
| Gene Summary | GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013] |
| Alternative Names | GABA A receptor subunit alpha 2 GABA GABA(A) receptor subunit alpha 2 GABA(A) receptor subunit alpha-2 GABR A2 GABRA 2 GABRA2 GABRA2 protein Gamma aminobutyric acid (GABA) A receptor alpha 2 Gamma aminobutyric acid A receptor alpha 2 Gamma aminobutyric acid receptor alpha 2 subunit Gamma aminobutyric acid receptor subunit alpha 2 Gamma-aminobutyric acid receptor subunit alpha-2 GBRA2_HUMAN |
| Molecular Weight(MW) | 51kDa |
| Cellular Localization | Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Cytoplasmic vesicle membrane. Cell projection>Dendrite. |
WB
Western blot analysis of extracts from Rat brain, using GABRA2 Antibody. The lane on the left was treated with blocking peptide.
ICC/IF
GABRA2 Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:500-1:2000 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
