WB
Western blot analysis of VTI1B on different lysates with Rabbit anti-VTI1B antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: MCF7 cell lysate (20 µg/Lane), Lane 3: U-2 OS cell lysate (20 µg/Lane), Lane 4: U-87 MG cell lysate (20 µg/Lane), Lane 5: SH-SY5Y cell lysate (20 µg/Lane), Lane 6: C2C12 cell lysate (20 µg/Lane), Lane 7: PC-12 cell lysate (20 µg/Lane), Lane 8: C6 cell lysate (20 µg/Lane), Lane 9: Rat brain tissue lysate (40 µg/Lane), Lane 10: Rat kidney tissue lysate (40 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-VTI1B antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of PC-12 cells labeling VTI1B with Rabbit anti-VTI1B antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VTI1B antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of PC-12 cells labeling VTI1B. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | VTI1B Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human VTI1B aa 1-232. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000 IHC:1:2000 ICC:1:100 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mgh/ml |
| Purification | Protein A |
| Gene Symbol | VTI1B |
|---|---|
| Gene Synonyms | VTI1 VTI2 VTI1L v-SNARE vti1-rp1 VTI1-LIKE |
| Gene Full Name | vesicle transport through interaction with t-SNAREs 1B |
| Gene Summary | Enables SNARE binding activity and chloride channel inhibitor activity. Involved in regulation of protein localization to plasma membrane. Located in several cellular components, including endosome membrane; lysosomal membrane; and perinuclear region of cytoplasm. [provided by Alliance of Genome Resources, Jan 2025] |
| Alternative Names | HGNC:17793 antibody V SNARE antibody v-SNARE antibody Vesicle associated soluble NSF attachment protein receptor antibody Vesicle transport through interaction with t SNAREs 1B antibody Vesicle transport through interaction with t SNAREs homolog 1B (yeast) antibody Vesicle transport through interaction with t-SNAREs homolog 1B antibody Vesicle transport v-SNARE protein Vti1-like 1 antibody VTI1 antibody VTI1 like antibody HGNC:17793 antibody V SNARE antibody v-SNARE antibody Vesicle associated soluble NSF attachment protein receptor antibody Vesicle transport through interaction with t SNAREs 1B antibody Vesicle transport through interaction with t SNAREs homolog 1B (yeast) antibody Vesicle transport through interaction with t-SNAREs homolog 1B antibody Vesicle transport v-SNARE protein Vti1-like 1 antibody VTI1 antibody VTI1 like antibody Vti1 rp1 antibody VTI1-LIKE antibody Vti1-rp1 antibody VTI1B antibody VTI1B_HUMAN antibody VTI1L antibody VTI1L1 antibody VTI2 antibody |
| Molecular Weight(MW) | 27kDa |
| Cellular Localization | Early endosome membrane, Late endosome membrane, Lysosome membrane, Cytoplasmic granule, Recycling endosome membrane. |
WB
Western blot analysis of VTI1B on different lysates with Rabbit anti-VTI1B antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: MCF7 cell lysate (20 µg/Lane), Lane 3: U-2 OS cell lysate (20 µg/Lane), Lane 4: U-87 MG cell lysate (20 µg/Lane), Lane 5: SH-SY5Y cell lysate (20 µg/Lane), Lane 6: C2C12 cell lysate (20 µg/Lane), Lane 7: PC-12 cell lysate (20 µg/Lane), Lane 8: C6 cell lysate (20 µg/Lane), Lane 9: Rat brain tissue lysate (40 µg/Lane), Lane 10: Rat kidney tissue lysate (40 µg/Lane), Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-VTI1B antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of PC-12 cells labeling VTI1B with Rabbit anti-VTI1B antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VTI1B antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of PC-12 cells labeling VTI1B. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 IHC:1:2000 ICC:1:100 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
