WB
Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody at 1/5,000 dilution. Lane 1: Human brain tissue lysate (40 µg/Lane) Lane 2: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 3: Rat brain tissue lysate (no heat) (20 µg/Lane) Lane 4: Mouse cerebellum tissue lysate (70℃ heat) (20 µg/Lane) Lane 5: Rat cerebellum tissue lysate (20 µg/Lane) Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane) Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane) Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GABA B Receptor 1 antibody at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABA B Receptor 1 with Rabbit anti-GABA B Receptor 1 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Product Name | GABA B Receptor 1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human GABA B Receptor 1 aa 142-591 / 961. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:5000 IHC:1:1000-1:20000 IF-P:1:50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GABBR1 |
|---|---|
| Gene Synonyms | GB1 NEDLC GPRC3A GABABR1 GABBR1-3 |
| Gene Full Name | gamma-aminobutyric acid type B receptor subunit 1 |
| Gene Summary | This gene encodes a receptor for gamma-aminobutyric acid (GABA), which is the main inhibitory neurotransmitter in the mammalian central nervous system. This receptor functions as a heterodimer with GABA(B) receptor 2. Defects in this gene may underlie brain disorders such as schizophrenia and epilepsy. Alternative splicing generates multiple transcript variants, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jan 2016] |
| Alternative Names | dJ271M21.1.1 antibody dJ271M21.1.2 antibody FLJ92613 antibody GABA-B receptor 1 antibody GABA-B-R1 antibody GABA-BR1 antibody GABAB R1 antibody GABAB subunit 1c antibody GABABR1 antibody GABBR1 3 antibody dJ271M21.1.1 antibody dJ271M21.1.2 antibody FLJ92613 antibody GABA-B receptor 1 antibody GABA-B-R1 antibody GABA-BR1 antibody GABAB R1 antibody GABAB subunit 1c antibody GABABR1 antibody GABBR1 3 antibody GABBR1 antibody GABR1_HUMAN antibody Gamma aminobutyric acid (GABA) B receptor 1 antibody Gamma-aminobutyric acid type B receptor subunit 1 antibody Gb1 antibody GPRC3A antibody Seven transmembrane helix receptor antibody |
| Molecular Weight(MW) | 108kDa(Observed band size: 95-108kDa) |
| Cellular Localization | Cell membrane, Postsynaptic cell membrane, Cell projection, dendrite; Secreted. |
WB
Western blot analysis of GABA B Receptor 1 on different lysates with Rabbit anti-GABA B Receptor 1 antibody at 1/5,000 dilution. Lane 1: Human brain tissue lysate (40 µg/Lane) Lane 2: Mouse brain tissue lysate (no heat) (20 µg/Lane) Lane 3: Rat brain tissue lysate (no heat) (20 µg/Lane) Lane 4: Mouse cerebellum tissue lysate (70℃ heat) (20 µg/Lane) Lane 5: Rat cerebellum tissue lysate (20 µg/Lane) Lane 6: Mouse skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane) Lane 7: Rat skeletal muscle tissue lysate (no heat) (negative) (20 µg/Lane) Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-GABA B Receptor 1 antibody at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Immunofluorescence analysis of paraffin-embedded mouse cerebellum tissue labeling GABA B Receptor 1 with Rabbit anti-GABA B Receptor 1 antibody at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:5000 IHC:1:1000-1:20000 IF-P:1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
