WB
Western blot analysis of MMP-14 on different lysates with Rabbit anti-MMP-14 antibody at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: MEF cell lysate, Lane 3: L6 cell lysate, Lane 4: Mouse lung tissue lysate, Lane 5: Rat lung tissue lysate, Lysates/proteins at 20/40 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat prostate tissue with Rabbit anti-MMP-14 antibody at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
ICC staining of MMP-14 in BT-20 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).FC
Flow cytometric analysis of MMP-14 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Product Name | MMP-14 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human MMP14 aa 149-200 / 582. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:10000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MMP14 |
|---|---|
| Gene Synonyms | MMP-14 MMP-X1 MT-MMP MT1MMP MTMMP1 WNCHRS MT1-MMP MT-MMP 1 |
| Gene Full Name | matrix metallopeptidase 14 |
| Gene Summary | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008] |
| Alternative Names | Matrix metallopeptidase 14 (membrane inserted) antibody Matrix metalloproteinase 14 antibody Matrix metalloproteinase-14 antibody Membrane type 1 matrix metalloproteinase antibody Membrane type 1 metalloprotease antibody Membrane type matrix metalloproteinase 1 antibody Membrane-type matrix metalloproteinase 1 antibody Membrane-type-1 matrix metalloproteinase antibody MMP 14 antibody MMP X1 antibody Matrix metallopeptidase 14 (membrane inserted) antibody Matrix metalloproteinase 14 antibody Matrix metalloproteinase-14 antibody Membrane type 1 matrix metalloproteinase antibody Membrane type 1 metalloprotease antibody Membrane type matrix metalloproteinase 1 antibody Membrane-type matrix metalloproteinase 1 antibody Membrane-type-1 matrix metalloproteinase antibody MMP 14 antibody MMP X1 antibody MMP-14 antibody MMP-X1 antibody Mmp14 antibody MMP14_HUMAN antibody MMPX1 antibody MT MMP 1 antibody MT-MMP 1 antibody MT1 MMP antibody MT1-MMP antibody MT1MMP antibody MTMMP 1 antibody MTMMP1 antibody |
| Molecular Weight(MW) | 66kDa(Observed band size: 55kDa) |
| Cellular Localization | Membrane, Melanosome, Cytoplasm. |
WB
Western blot analysis of MMP-14 on different lysates with Rabbit anti-MMP-14 antibody at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: MEF cell lysate, Lane 3: L6 cell lysate, Lane 4: Mouse lung tissue lysate, Lane 5: Rat lung tissue lysate, Lysates/proteins at 20/40 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat prostate tissue with Rabbit anti-MMP-14 antibody at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
ICC staining of MMP-14 in BT-20 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
FC
Flow cytometric analysis of MMP-14 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:10000 ICC:1:50-1:200 FC:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
