WB
Western blot analysis of Insulin on different lysates with Rabbit anti-Insulin antibody (ET1601-12) at 1/1,000 dilution. Lane 1: Mouse pancreas tissue lysate Lane 2: Rat pancreas tissue lysate Lane 3: Rat colon tissue lysate (negative) Lysates/proteins at 40 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-12) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Insulin antibody at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Immunofluorescence analysis of frozen mouse pancreas tissue with Rabbit anti-Insulin antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).IF-P
Immunofluorescence analysis of paraffin-embedded human pancreas tissue labeling Insulin with Rabbit anti-Insulin antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).| Product Name | Insulin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Insulin aa 15-110. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIF-PIHCWB |
| WB:1:1000 IHC:1:20000 IF-F:1:1000 IF-P:1:200-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | INS |
|---|---|
| Gene Synonyms | IDDM ILPR IRDN IDDM1 IDDM2 PNDM4 MODY10 |
| Gene Full Name | insulin |
| Gene Summary | This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. [provided by RefSeq, May 2020] |
| Alternative Names | IDDM antibody IDDM1 antibody IDDM2 antibody ILPR antibody ins antibody INS_HUMAN antibody Insulin A chain antibody Insulin B chain antibody IRDN antibody MODY10 antibody IDDM antibody IDDM1 antibody IDDM2 antibody ILPR antibody ins antibody INS_HUMAN antibody Insulin A chain antibody Insulin B chain antibody IRDN antibody MODY10 antibody Preproinsulin antibody Proinsulin antibody Proinsulin precursor antibody |
| Molecular Weight(MW) | 12kDa |
| Cellular Localization | Secreted. |
WB
Western blot analysis of Insulin on different lysates with Rabbit anti-Insulin antibody (ET1601-12) at 1/1,000 dilution. Lane 1: Mouse pancreas tissue lysate Lane 2: Rat pancreas tissue lysate Lane 3: Rat colon tissue lysate (negative) Lysates/proteins at 40 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-12) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Insulin antibody at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Immunofluorescence analysis of frozen mouse pancreas tissue with Rabbit anti-Insulin antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
IF-P
Immunofluorescence analysis of paraffin-embedded human pancreas tissue labeling Insulin with Rabbit anti-Insulin antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:1000 IHC:1:20000 IF-F:1:1000 IF-P:1:200-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
