WB
Western blot analysis of recombinant Human CXCL9 Protein using CXCL9/MIG Protein Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 5ng/1ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.ICC/IF
Confocal imaging of 293T cells transfected with CXCL9/MIG using CXCL9/MIG Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L)(dilution 1:500)(Red). DAPI was used for nuclear staining (Blue).objective: 100x.| Product Name | CXCL9/MIG Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 23-125 of human CXCL9/MIG( NP_002407.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:4000 ICC:1:200-1:800 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXCL9 |
|---|---|
| Gene Synonyms | CMK MIG Humig SCYB9 crg-10 |
| Gene Full Name | C-X-C motif chemokine ligand 9 |
| Gene Summary | This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded is thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils. [provided by RefSeq, Aug 2020] |
| Alternative Names | CMK MIG Humig SCYB9 crg-10 CXCL9 |
| Molecular Weight(MW) | 14kDa(Observed MW 35kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of recombinant Human CXCL9 Protein using CXCL9/MIG Protein Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 5ng/1ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
ICC/IF
Confocal imaging of 293T cells transfected with CXCL9/MIG using CXCL9/MIG Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L)(dilution 1:500)(Red). DAPI was used for nuclear staining (Blue).objective: 100x.| Application Notes | WB:1:1000-1:4000 ICC:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
