WB
Western blot analysis of Galectin 1 on different lysates with Rabbit anti-Galectin 1 antibody at 1/2,000 dilution. Lane 1: HL-60 cell lysate, Lane 2: LNCaP cell lysate (negative), Lane 3: A549 cell lysate, Lane 4: HCT 116 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Galectin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling Galectin 1 with Rabbit anti-Galectin 1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Galectin 1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of L6 cells labeling Galectin 1. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Galectin 1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Galectin 1 aa 1-50 / 135. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000 IHC:1:400-1:800 ICC:1:50-1:200 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LGALS1 |
|---|---|
| Gene Synonyms | GBP GAL1 |
| Gene Full Name | galectin 1 |
| Gene Summary | The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008] |
| Alternative Names | 14 kDa laminin-binding protein antibody 14 kDa lectin antibody Beta galactoside binding lectin antibody Beta galactoside binding lectin L 14 I antibody beta galactoside binding protein antibody Beta-galactoside-binding lectin L-14-I antibody DKFZp686E23103 antibody Gal 1 antibody Gal-1 antibody GAL1 antibody 14 kDa laminin-binding protein antibody 14 kDa lectin antibody Beta galactoside binding lectin antibody Beta galactoside binding lectin L 14 I antibody beta galactoside binding protein antibody Beta-galactoside-binding lectin L-14-I antibody DKFZp686E23103 antibody Gal 1 antibody Gal-1 antibody GAL1 antibody Galaptin antibody Galbp antibody Galectin antibody Galectin-1 antibody Galectin1 antibody GBP antibody HBL antibody HLBP14 antibody HPL antibody L 14.5 antibody L-14.5 antibody L14 antibody Lactose binding lectin 1 antibody Lactose-binding lectin 1 antibody Lect14 antibody Lectin galactoside binding soluble 1 antibody Lectin galactoside-binding soluble 1 antibody LEG1_HUMAN antibody LGALS 1 antibody LGALS1 antibody Lgals1 lectin galactose binding soluble 1 antibody MAPK activating protein MP12 antibody Putative MAPK activating protein MP12 antibody Putative MAPK-activating protein PM12 antibody S Lac lectin 1 antibody S-Lac lectin 1 antibody |
| Molecular Weight(MW) | 14kDa |
| Cellular Localization | Cytoplasm, Extracellular matrix, Secreted. |
WB
Western blot analysis of Galectin 1 on different lysates with Rabbit anti-Galectin 1 antibody at 1/2,000 dilution. Lane 1: HL-60 cell lysate, Lane 2: LNCaP cell lysate (negative), Lane 3: A549 cell lysate, Lane 4: HCT 116 cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Galectin 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling Galectin 1 with Rabbit anti-Galectin 1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Galectin 1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of L6 cells labeling Galectin 1. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000 IHC:1:400-1:800 ICC:1:50-1:200 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
