WB
Western blot analysis of ATG16L1 on different lysates with Rabbit anti-ATG16L1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: Jurkat cell lysate, Lane 3: RAW264.7 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-ATG16L1 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | ATG16L1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within N-terminal Human Atg16L1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ATG16L1 |
|---|---|
| Gene Synonyms | IBD10 WDR30 APG16L ATG16A ATG16L |
| Gene Full Name | autophagy related 16 like 1 |
| Gene Summary | The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010] |
| Alternative Names | A16L1_HUMAN antibody APG16 like 1 antibody APG16-like 1 antibody APG16L antibody APG16L beta antibody ATG16 autophagy related 16 like 1 antibody ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody ATG16A antibody ATG16L antibody Atg16l1 antibody A16L1_HUMAN antibody APG16 like 1 antibody APG16-like 1 antibody APG16L antibody APG16L beta antibody ATG16 autophagy related 16 like 1 antibody ATG16 autophagy related 16-like 1 (S. cerevisiae) antibody ATG16A antibody ATG16L antibody Atg16l1 antibody Autophagy related protein 16 1 antibody Autophagy-related protein 16-1 antibody FLJ00045 antibody FLJ10035 antibody FLJ10828 antibody FLJ22677 antibody IBD10 antibody OTTHUMP00000164391 antibody OTTHUMP00000164393 antibody OTTHUMP00000165876 antibody OTTHUMP00000165877 antibody WD repeat domain 30 antibody WDR30 antibody |
| Molecular Weight(MW) | 68kDa(Observed band size: 68/66 kDa) |
| Cellular Localization | Cytoplasm. |
WB
Western blot analysis of ATG16L1 on different lysates with Rabbit anti-ATG16L1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: Jurkat cell lysate, Lane 3: RAW264.7 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-ATG16L1 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:2000 IHC:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
