WB
Various lysates were subjected to SDS PAGE followed by western blot with MSH2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using MSH2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH2 antibody at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L), CL594-Phalloidin (red).IP
IP result of anti-MSH2 (IP:5ug; Detection: 1:500) with HeLa cells lysate 2000ug.FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MSH2 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.| Product Name | MSH2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human MSH2 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:500 ICC:1:400-1:1600 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate FC: 0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MSH2 |
|---|---|
| Gene Synonyms | COCA1 FCC1 HNPCC HNPCC1 LCFS2 LYNCH1 MMRCS2 MSH-2 hMSH2 |
| Gene Full Name | mutS homolog 2 |
| Target Description | MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell. |
| Alternative Names | MSH 2 |
| Molecular Weight(MW) | 100kDa-105kDa |
| Cellular Localization | Nucleus,Chromosome. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with MSH2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using MSH2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MSH2 antibody at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L), CL594-Phalloidin (red).
IP
IP result of anti-MSH2 (IP:5ug; Detection: 1:500) with HeLa cells lysate 2000ug.
FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MSH2 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:500 ICC:1:400-1:1600 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate FC: 0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
