WB
Western blot analysis of PTGER2 on different lysates with Rabbit anti-PTGER2 antibody at 1/1,000 dilution. Lane 1: JAR cell lysate (15 µg/Lane) Lane 2: NIH/3T3 cell lysate (15 µg/Lane) Lane 3: Human placenta tissue lysate (30 µg/Lane) Lane 4: Mouse placenta tissue lysate (30 µg/Lane) Lane 5: Mouse lung tissue lysate (30 µg/Lane) Lane 6: Rat lung tissue lysate (30 µg/Lane) Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PTGER2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of JAR cells labeling PTGER2 with Rabbit anti-PTGER2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PTGER2 antibody at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of JAR cells labeling PTGER2. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | PTGER2 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human PTGER2 aa 300-358 / 358. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000 IHC:1:200-1:1000 ICC:1:100 FC:1:500-1:1000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PTGER2 |
|---|---|
| Gene Synonyms | EP2 |
| Gene Full Name | prostaglandin E receptor 2 |
| Gene Summary | This gene encodes a receptor for prostaglandin E2, a metabolite of arachidonic acid which has different biologic activities in a wide range of tissues. Mutations in this gene are associated with aspirin-induced susceptibility to asthma. [provided by RefSeq, Oct 2009] |
| Alternative Names | EP2 antibody PE2R2_HUMAN antibody PGE receptor EP2 subtype antibody PGE2 receptor EP2 subtype antibody Prostaglandin E receptor 2 EP2 subtype antibody Prostaglandin E receptor 2 subtype EP2 53kDa antibody Prostaglandin E receptor 2 subtype EP2 antibody Prostaglandin E2 receptor antibody Prostaglandin E2 receptor EP2 subtype antibody Prostanoid EP2 receptor antibody EP2 antibody PE2R2_HUMAN antibody PGE receptor EP2 subtype antibody PGE2 receptor EP2 subtype antibody Prostaglandin E receptor 2 EP2 subtype antibody Prostaglandin E receptor 2 subtype EP2 53kDa antibody Prostaglandin E receptor 2 subtype EP2 antibody Prostaglandin E2 receptor antibody Prostaglandin E2 receptor EP2 subtype antibody Prostanoid EP2 receptor antibody Ptger2 antibody |
| Molecular Weight(MW) | 40kDa(Observed band size: 50kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of PTGER2 on different lysates with Rabbit anti-PTGER2 antibody at 1/1,000 dilution. Lane 1: JAR cell lysate (15 µg/Lane) Lane 2: NIH/3T3 cell lysate (15 µg/Lane) Lane 3: Human placenta tissue lysate (30 µg/Lane) Lane 4: Mouse placenta tissue lysate (30 µg/Lane) Lane 5: Mouse lung tissue lysate (30 µg/Lane) Lane 6: Rat lung tissue lysate (30 µg/Lane) Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PTGER2 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of JAR cells labeling PTGER2 with Rabbit anti-PTGER2 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PTGER2 antibody at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of JAR cells labeling PTGER2. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 IHC:1:200-1:1000 ICC:1:100 FC:1:500-1:1000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
