WB
Western blot analysis of Beta-2 Microglobulin on different lysates with Rabbit anti-Beta-2 Microglobulin antibody at 1/5,000 dilution. Lane 1: Mouse spleen tissue lysate, Lane 2: Mouse liver tissue lysate, Lane 3: Rat spleen tissue lysate, Lane 4: Rat liver tissue lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
ICC staining of Beta-2 Microglobulin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).ICC/IF
ICC staining of Beta-2 Microglobulin in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).| Product Name | Beta-2 Microglobulin Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within C-terminal human beta 2 Microglobulin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:5000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | B2M |
|---|---|
| Gene Synonyms | IMD43 AMYLD6 MHC1D4 |
| Gene Full Name | beta-2-microglobulin |
| Gene Summary | This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014] |
| Alternative Names | B2M antibody B2MG_HUMAN antibody Beta 2 microglobin antibody Beta 2 microglobulin antibody Beta 2 microglobulin precursor antibody Beta chain of mhc class 1 proteins antibody Beta chain of MHC class I molecules antibody Beta-2-microglobulin form pI 5.3 antibody CDABP0092 antibody Hdcma22p antibody B2M antibody B2MG_HUMAN antibody Beta 2 microglobin antibody Beta 2 microglobulin antibody Beta 2 microglobulin precursor antibody Beta chain of mhc class 1 proteins antibody Beta chain of MHC class I molecules antibody Beta-2-microglobulin form pI 5.3 antibody CDABP0092 antibody Hdcma22p antibody IMD43 antibody |
| Molecular Weight(MW) | 12kDa |
| Cellular Localization | Secreted. |
WB
Western blot analysis of Beta-2 Microglobulin on different lysates with Rabbit anti-Beta-2 Microglobulin antibody at 1/5,000 dilution. Lane 1: Mouse spleen tissue lysate, Lane 2: Mouse liver tissue lysate, Lane 3: Rat spleen tissue lysate, Lane 4: Rat liver tissue lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
ICC staining of Beta-2 Microglobulin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ICC/IF
ICC staining of Beta-2 Microglobulin in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:1000-1:5000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
