WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-DRP1 antibody. The HRP conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat brain, Lane 2: Mouse brain.
IHC
Rat brain was stained with Anti-DRP1 rabbit antibody.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,primary Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.